Donkey anti-Sheep IgG (H&L), HRP conjugated
SKU: BTL-AGR-A-00379 | Host: Donkey | Brand: Agrisera
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| Cat Number | AS09 604 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 1 mg |
| Description | Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays. |
| Applications | ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB) |
| Immunogen | Purified sheep IgG, whole molecule |
| Clonality | Polyclonal |
| Host | Donkey |
| Form | Lyophilized |
| Dilution Range | 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC) |
| Purity | Immunogen affinity purified donkey IgG. |
| Specie Reactivity | Sheep IgG heavy chains and light chains on all sheep immunoglobulins |
| Reconstitution | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily |
| Storage | Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
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| Note | The product is for research use only |