Goat anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)
SKU: BTL-AGR-A-00392 | Host: Goat | Brand: Agrisera
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| Cat Number | AS09 618 |
|---|---|
| Category | Secondary Antibody |
| Pack Size | 1 mg |
| Description | Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays. |
| Applications | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
| Immunogen | Purified Rat IgG, whole molecule |
| Clonality | Polyclonal |
| Host | Goat |
| Form | Lyophilized |
| Dilution Range | 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB) |
| Purity | Immunogen affinity purified goat IgG. |
| Specie Reactivity | Rat IgG heavy and light chains (H&L) of all Rat immunoglobulins |
| Expected Reactivity | Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins |
| Reconstitution | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily |
| Storage | Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
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| Note | The product is for research use only |