Rabbit anti-Chicken IgY (H&L), HRP conjugated
SKU: BTL-AGR-A-00689 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS10 1489 |
|---|---|
| Category | Secondary Antibody |
| Pack Size | 1 mg |
| Description | Rabbit anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP, which binds to in immunological assays. Antibody is affinity purified using solid phase chicken IgY.Chicken egg yolk immunoglobulin (IgY) has been also called chicken IgG. |
| Applications | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
| Immunogen | Purified Chicken IgY (H&L), whole molecule |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Lyophilized |
| Dilution Range | The optimal working dilution should be determined by the investigator |
| Purity | Immunogen affinity purified rabbit IgG. |
| Specie Reactivity | Heavy chains on chicken IgY and light chains on all chicken immunoglobulins, |
| Reconstitution | For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily |
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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| Note | The product is for research use only |