SKU: BTL-AGR-A-01422 | Host: Mouse | Brand: Agrisera
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| Cat Number | AS15 2871 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 50 µg |
| Description | FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits. |
| Applications | Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB) |
| Immunogen | KLH-conjugated DYKDDDDK (FLAG) synthetic peptide |
| Clonality | Monoclonal |
| Host | Mouse |
| Form | Liquid |
| Dilution Range | 1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user |
| Purity | Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide. |
| Specie Reactivity | N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins |
| Storage | Store at -20°C for up to 2 years. |
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| Product Url | View Document |
| Note | The product is for research use only |