Home / Antibody / Anti-DYKDDDDK (binds to Sigma FLAG®, clone FG4R) , 50 µg
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Anti-DYKDDDDK (binds to Sigma FLAG®, clone FG4R)

SKU: BTL-AGR-A-01422 | Host: Mouse | Brand: Agrisera

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Cat Number AS15 2871
Category Primary Antibody
Pack Size 50 µg
Description FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
Applications Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Immunogen KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
Clonality Monoclonal
Host Mouse
Form Liquid
Dilution Range 1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user
Purity Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Specie Reactivity N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Storage Store at -20°C for up to 2 years.
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