SKU: BTL-AGR-A-01604 | Host: Goat | Brand: Agrisera
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| Cat Number | AS16 3129 |
|---|---|
| Category | Secondary Antibody |
| Pack Size | 10 mg |
| Description | Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays. |
| Applications | Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB) |
| Immunogen | Purified normal IgM isolated from pooled dog serum |
| Clonality | Polyclonal |
| Host | Goat |
| Form | Lyophilized |
| Dilution Range | 1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal |
| Purity | Protein G purified goat IgG in PBS pH 7.2. |
| Specie Reactivity | Dog IgM, Fc part |
| Reconstitution | For reconstitution, add 1 ml of sterile distilled water, spin down to remove any insoluble particles. |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be prepared by adding sterile PBS pH 7.2. Working dilutions should be stored at 4°C, not frozen, and preferably used during the same day. If any precipitation occurs, remove it by centrifugation. |
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| Note | The product is for research use only |