Anti-5-hmC | 5-hydroxymethylcytosine (monoclonal)
SKU: BTL-AGR-A-01632 | Host: Mouse | Brand: Agrisera
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| Cat Number | AS16 3164 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 100 µg |
| Description | 5-hmC | 5-hydroxymethylcytosine is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. It may have an important roles distinct from that of 5-methylcytosine (5-mC). 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells. |
| Applications | Dot blot (Dot), ELISA (ELISA), hMeDIP (hydroxymethylated DNA Immunoprecipitation (IP) |
| Immunogen | BSA-conjugated molecule: 5-hydroxymethylcytosine (5-hmC). |
| Clonality | Monoclonal |
| Host | Mouse |
| Form | Liquid |
| Dilution Range | 2 µg/ml (Dot), 1 : 500 (ELISA), 2,5 µg/IP (hMeDIP) |
| Purity | Protein G purified. |
| Specie Reactivity | Human |
| Expected Reactivity | Mouse, broad species range |
| Storage | Store lyophilized/reconstituted at -20°C; for long term storage -80°C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |