Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal)
SKU: BTL-AGR-A-01867 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS16 3690 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 50 µg |
| Description | Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. |
| Applications | Immunolocalization (IL), Western blot (WB) |
| Immunogen | KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Lyophilized |
| Molecular Weight | Depends on the MW of the protein that is the fusion partner. |
| Dilution Range | 1 : 100 (IL), 1 : 1000 (WB) |
| Uniprot Number | Q03JI6 |
| Purity | Immunogen affinity purified serum in PBS pH 7.4. |
| Specie Reactivity | Staphylococcus aureus |
| Expected Reactivity | Staphylococcus pyogenes UniProt: Q99ZW2 |
| Reconstitution | For reconstitution add 50 µl of sterile water |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |