Anti-BirA (mutated/TurboID) - DyLight® 488 conjugated (40 µg)
SKU: BTL-AGR-A-02228 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS20 4440-DL488 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 40 µg |
| Description | Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa. |
| Applications | Immunofluorescence (IF), Western blot (WB) |
| Immunogen | Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Liquid in PBS pH 7,4. |
| Molecular Weight | Depends upon fusion partner |
| Dilution Range | To be determined by end user. |
| Purity | Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 488. |
| Specie Reactivity | BirA (mutated/TurboID) |
| Storage | Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use. |
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| Note | The product is for research use only |