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Anti-His-tag | 3xHis (polyclonal)

SKU: BTL-AGR-A-02233 | Host: Rabbit | Brand: Agrisera

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Cat Number AS20 4441
Category Primary Antibody
Pack Size 50 µg
Description His-Tag is a polyhistidine tag which consists of 3 or 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Applications Immunofluorescence (IF), Western blot (WB)
Immunogen KLH-conjugated synthetic peptide 3xHis
Clonality Polyclonal
Host Rabbit
Form Lyophilized
Molecular Weight 3xHis tagged proteins
Dilution Range 1 μg/mL (IF), 1: 1000 - 1 : 5000 (WB)
Uniprot Number UniProt
Purity Antigen affinity purified serum in PBS pH 7.4.
Specie Reactivity 3xHis tagged proteins
Reconstitution For reconstitution add 50 µl, of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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